Direct detection of insulin sensitivity

2019-09-04
 
Basic operation method: 12 h on an empty stomach overnight, measuring height and weight, waking up after urination, and lying on the examination bed for 30 min to start the test. The infusion channel was established by puncture and indwelling catheters on both sides of the subject's forearm or median vein, one for insulin and glucose solution, and the other for blood sampling in the test (put this side forearm in a 50 °C incubator) To ensure venous blood arterialization, maintain the passage with saline. The basal blood glucose level of the subject was measured and set as the clamp target (4.4~5.0 mmol/L). The insulin dose (45 mU/m2) was administered to the subject within 10 min before the clamp test, and the plasma was rapidly increased. Insulin levels inhibit hepatic glucose output and endogenous insulin secretion (generally 100 mU/L), followed by continuous infusion at a rate of 40 mU·(m2)-1·min-1 to obtain stable high-concentration plasma insulin Level. During this period, the blood glucose level is measured once every 5 minutes, the infusion rate according to the blood glucose level is adjusted and 20% glucose is adjusted, the blood sugar is maintained at the target level, and the blood glucose tends to be stable when the blood glucose is stabilized; every 10~30 Blood samples were taken for minute glucose, insulin, C-peptide, cortisol, growth hormone, glucagon, and free fatty acids. All blood samples were separated by centrifugation of plasma or serum and stored at -70 °C until assayed. The entire test process lasted for 2.0 to 2.5 hours.
 
Excerpt from: Knowing - Flying Brother talk